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Cropit scale quality8/14/2023 ![]() ![]() Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Chimeric nucleases stimulate gene targeting in human cells. Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. A TALE nuclease architecture for efficient genome editing. A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. High-efficiency multiplex genome editing of streptomyces species using an engineered CRISPR/Cas system. Search-and-replace genome editing without double-strand breaks or donor DNA. Base editing: precision chemistry on the genome and transcriptome of living cells. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. CRISPR–Cas systems for editing, regulating and targeting genomes. Genome engineering with zinc-finger nucleases. TALENs: a widely applicable technology for targeted genome editing. Therapeutic genome editing: prospects and challenges. High prevalence of Streptococcus pyogenes Cas9-reactive T cells within the adult human population. Identification of preexisting adaptive immunity to Cas9 proteins in humans. Prevalence of pre-existing antibodies to CRISPR-associated nuclease Cas9 in the USA population. AAV-CRISPR gene editing is negated by pre-existing immunity to Cas9. Engineered materials for in vivo delivery of genome-editing machinery. DNA targeting specificity of RNA-guided Cas9 nucleases. High-frequency off-target mutagenesis induced by CRISPR–Cas nucleases in human cells. CRISPR/Cas9 systems targeting beta-globin and CCR5 genes have substantial off-target activity. Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR–Cas nucleases. Mutation detection using Surveyor nuclease. Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly. Easy quantitative assessment of genome editing by sequence trace decomposition. ![]() Inference of CRISPR edits from Sanger trace data. Examining sources of error in PCR by single-molecule sequencing. Somatic genome editing with CRISPR/Cas9 generates and corrects a metabolic disease. ![]()
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